Name: Canine EGF-Protein (EGF-DE101)
Brand: KactusBio
SKU: EGF-DE101-100UG
Price: 379.5 USD
Availability: InStock

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Canine EGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test).
Amino Acid Range: Asn973-Arg1024

Catalog #EGF-DE101

Size

100UG

500UG

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Canine EGF-Protein (EGF-DE101) Summary

Product Description	Recombinant Canine EGF-Protein is expressed from E.coli with His tag at the N-terminus.It contains Asn973-Arg1024.
Product Background	The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Canine EGF-Protein (EGF-DE101) Specifications/Details

Protein	EGF
Synonyms	HOMG4; URG
Accession	Q9BEA0
Species	Canine
Biotinylated	no
Amino Acid Range	Asn973-Arg1024
Molecular Weight	The protein has a predicted MW of 13.61 kDa. The protein migrates to 16-22 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Canine EGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test).
Product Tag	N-His
Fluorophore	Unconjugated
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Canine EGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test).
	Canine EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using Balb 3T3 mouse embryonic fibroblasts. The EC50-for this effect is typically 0.5 - 3 ng/mL.
	The purity of Canine EGF is greater than 95% as determined by SEC-HPLC.

Other Available Proteins:

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