Canine EGF-Protein (EGF-DE101)
Canine EGF-Protein (EGF-DE101)
  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Canine EGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Asn973-Arg1024
Catalog #EGF-DE101
Regular price $345.00
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Product Description
Recombinant Canine EGF-Protein is expressed from E.coli with His tag at the N-terminus.It contains Asn973-Arg1024.
Product Background
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Product Category
Recombinant Protein
Target
EGF
Accession
Species
Canine
Biotinylated
no
Amino Acid Range
Asn973-Arg1024
Molecular Weight
The protein has a predicted MW of 13.61 kDa. The protein migrates to 16-22 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Canine EGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
N-His
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Canine EGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test).
Canine EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using Balb 3T3 mouse embryonic fibroblasts. The EC50-for this effect is typically 0.5 - 3 ng/mL.
The purity of Canine EGF is greater than 95% as determined by SEC-HPLC.

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