• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 40000 pmol/min/ug.
  • Amino Acid Range: Leu88-Asp916
Catalog #ENP-DM102
Price
Regular price $37500
Regular price Sale price $37500
/
Size
Avantor VWR Fisher Scientific

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Canine ENPP-1 Protein (ENP-DM102) Summary

Product Description

 Recombinant Canine ENPP-1 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Leu88-Asp916.

Product Background

 Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.

Canine ENPP-1 Protein (ENP-DM102) Specifications/Details

Protein
 ENPP-1
Synonyms
 E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2;Ly41 Antigen
Accession
Species
 Canine
Biotinylated
 no
Amino Acid Range
 Leu88-Asp916
Molecular Weight
 The protein has a predicted MW of 96.4 kDa. Due to glycosylation, the protein migrates to 104-120 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 40000 pmol/min/ug.
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Canine ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Canine ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Other Available Proteins:
Finding available proteins...