Canine ENPP-1 Protein (ENP-DM102)

Express System: HEK293
Product Tag: N-His
Purity: > 90% as determined by Bis-Tris PAGE
Activity: Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 40000 pmol/min/ug.
Amino Acid Range: Leu88-Asp916

Catalog #ENP-DM102

Price

$375.00

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Canine ENPP-1 Protein (ENP-DM102) Summary

Product Description	Recombinant Canine ENPP-1 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Leu88-Asp916.
Product Background	Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	ENPP-1
Synonyms	E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2
Accession	A0A8C0PJE1
Species	Canine
Biotinylated	no
Amino Acid Range	Leu88-Asp916
Molecular Weight	The protein has a predicted MW of 96.4 kDa. Due to glycosylation, the protein migrates to 104-120 kDa based on Bis-Tris PAGE result.
Activity	Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 40000 pmol/min/ug.
Product Tag	N-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Canine ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

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