Product Description |
Recombinant Canine IL-13 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Ser19-Arg131. |
Product Background |
Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity). |
Product Category |
Recombinant Protein |
Target |
IL-13 |
Synonyms |
IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; |
Accession |
|
Species |
Canine |
Biotinylated |
no |
Amino Acid Range |
Ser19-Arg131 |
Molecular Weight |
The protein has a predicted MW of 38.38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Canine IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Canine IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). |
|
|
Canine IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Canine IL-13 is greater than 95% as determined by SEC-HPLC. |
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