Name: Canine IL-13 Protein (IL1-DM213)
Brand: KactusBio
SKU: IL1-DM213-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Canine IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ser19-Arg131

Catalog #IL1-DM213

Size

100UG

500UG

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Canine IL-13 Protein (IL1-DM213) Summary

Product Description	Recombinant Canine IL-13 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Ser19-Arg131.
Product Background	Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Canine IL-13 Protein (IL1-DM213) Specifications/Details

Protein	IL-13
Synonyms	IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1;
Accession	Q9N0W9
Species	Canine
Biotinylated	no
Amino Acid Range	Ser19-Arg131
Molecular Weight	The protein has a predicted MW of 38.38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Canine IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Canine IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
	Canine IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Canine IL-13, hFc Tag on ProA-Biosensor can bind Human IL-13Ra2, His Tag with an affinity constant of 2.07 nM as determined in BLI assay (Gator® Prime).
	The purity of Canine IL-13 is greater than 95% as determined by SEC-HPLC.