SDS-PAGE gel image of recombinant canine IL-13 protein, showing molecular weight validation
Canine IL-13 Protein (IL1-DM213)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Canine IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ser19-Arg131
Catalog #IL1-DM213
Regular price $346.50
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Canine IL-13 Protein (IL1-DM213) Summary

Product Description

Recombinant Canine IL-13 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Ser19-Arg131.

Product Background

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Canine IL-13 Protein (IL1-DM213) Specifications/Details

Protein
IL-13
Synonyms
IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1;
Accession
Species
Canine
Biotinylated
no
Amino Acid Range
Ser19-Arg131
Molecular Weight
The protein has a predicted MW of 38.38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Canine IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Canine IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). Immobilized Canine IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
Canine IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Canine IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Canine IL-13, hFc Tag on ProA-Biosensor can bind Human IL-13Ra2, His Tag with an affinity constant of 2.07 nM as determined in BLI assay (Gator® Prime). Loaded Canine IL-13, hFc Tag on ProA-Biosensor can bind Human IL-13Ra2, His Tag with an affinity constant of 2.07 nM as determined in BLI assay (Gator® Prime).
The purity of Canine IL-13 is greater than 95% as determined by SEC-HPLC. The purity of Canine IL-13 is greater than 95% as determined by SEC-HPLC.
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