Canine LIF R/CD118 Protein, Ultra Low Endotoxin (LIF-DM10R-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LIF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Canine LIF R, His Tag with the EC50 of 25.4ng/ml determined by ELISA.
  • Amino Acid Range: Glu45-Ser833
Catalog #LIF-DM10R-UL
Regular price $417.00
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Canine LIF R/CD118 Protein, Ultra Low Endotoxin (LIF-DM10R-UL) Summary

Product Description

Recombinant Canine LIF R/CD118 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu45-Ser833.

Product Background

The leukemia inhibitory factor receptor (LIF-R) subunit is a component of cell-surface receptor complexes for the multifunctional cytokines, LIF, cardiotrophin-1, ciliary neurotrophic factor, and human oncostatin M. The structure of the human LIF-R gene is similar to that of the mouse gene. The transmembrane receptor is encoded by 19 exons.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Canine LIF R/CD118 Protein, Ultra Low Endotoxin (LIF-DM10R-UL) Specifications/Details

Protein
LIF R/CD118
Synonyms
FLJ99923; SJS2; STWS; SWS; LIFR; CD118; FLJ98106
Accession
Species
Canine
Biotinylated
no
Amino Acid Range
Glu45-Ser833
Molecular Weight
The protein has a predicted MW of 91.21 kDa. Due to glycosylation, the protein migrates to 120-150 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human LIF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Canine LIF R, His Tag with the EC50 of 25.4ng/ml determined by ELISA.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human LIF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Canine LIF R, His Tag with the EC50 of 25.4ng/ml determined by ELISA. Immobilized Human LIF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Canine LIF R, His Tag with the EC50 of 25.4ng/ml determined by ELISA.
Canine LIF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Canine LIF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Canine LIF R is greater than 95% as determined by SEC-HPLC. The purity of Canine LIF R is greater than 95% as determined by SEC-HPLC.
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