Product Description |
Recombinant Canine PDGF R beta/CD140b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly32-Val532. |
Product Background |
PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. |
Product Category |
Recombinant Protein |
Target |
PDGF R beta/CD140b |
Accession |
|
Species |
Canine |
Biotinylated |
no |
Amino Acid Range |
Gly32-Val532 |
Molecular Weight |
The protein has a predicted MW of 57.25 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.203 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human PDGFB, His Tag immobilized on CM5 Chip can bind Canine PDGF R beta, His Tag with an affinity constant of 0.203 uM as determined in SPR assay (Biacore T200). |
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|
Canine PDGF R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Canine PDGF R beta is greater than 95% as determined by SEC-HPLC. |
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