Product Description |
Recombinant Canine PDGFA Protein is expressed from Yeast with His tag at the N-terminus.It contains Ser87-Arg196. |
Product Background |
Platelet-derived growth factors (PDGFs) may play an important role in the development of atherosclerosis acting as chemoattractants and mitogens for vascular smooth muscle cells and macrophages. PDGFA, the most known member of PDGF family, plays a crucial role in occurrence and progression of different tumors. |
Product Category |
Recombinant Protein |
Target |
PDGFA |
Accession |
|
Species |
Canine |
Biotinylated |
no |
Amino Acid Range |
Ser87-Arg196 |
Molecular Weight |
The protein has a predicted MW of 13.62 kDa. Due to glycosylation, the protein migrates to 15-20 kDa and 35-45 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Express System |
Yeast |
Purity |
> 90% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCL. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Canine PDGFA on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
|
|
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is 5 - 15 ng/mL. |
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