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Canine RNF43 Protein (RNF-DM134) Summary
Product Description |
Recombinant Canine RNF43 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly24-Tyr197. |
Product Background |
RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Canine RNF43 Protein (RNF-DM134) Specifications/Details
Protein |
RNF43 |
Synonyms |
E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43 |
Accession |
|
Species |
Canine |
Biotinylated |
no |
Amino Acid Range |
Gly24-Tyr197 |
Molecular Weight |
The protein has a predicted MW of 20.68 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4); Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Canine RNF43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Canine RNF43 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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