Canine VEGF164 Protein (VEG-DM464)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Canine VEGF164, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Bevacizumab, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
  • Amino Acid Range: Ala27-Arg190
Catalog #VEG-DM464
Regular price $345.00
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Size
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Canine VEGF164 Protein (VEG-DM464) Summary

Product Description

Recombinant Canine VEGF164 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Ala27-Arg190.

Product Background

Vascular endothelial growth factor (VEGF) is an essential regulator of vascularization. It is expressed as several splice variants; the major forms contain 120 amino acids, 164 amino acids, and 188 amino acids. VEGF-A164 induces an extremely heterogeneous response that includes vascular hyperpermeability, extravascular fibrin deposition, edema, angiogenesis (mother vessels, GB, daughter capillaries), arteriovenous malformations, arteriogenesis, fibrogenesis, and lymphangiogenesis.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Canine VEGF164 Protein (VEG-DM464) Specifications/Details

Protein
VEGF164
Synonyms
VEGF; VEGFA; VEGF-A; VPF
Accession
Species
Canine
Biotinylated
no
Amino Acid Range
Ala27-Arg190
Molecular Weight
The protein has a predicted MW of 22.05 kDa. Due to glycosylation, the protein migrates to 27-33 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Canine VEGF164, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Bevacizumab, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
Product Tag
N-His-Avi

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Canine VEGF164, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Bevacizumab, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. Immobilized Canine VEGF164, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Bevacizumab, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
Canine VEGF164 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Canine VEGF164 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Canine VEGF164 is greater than 90% as determined by SEC-HPLC. The purity of Canine VEGF164 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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