Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM150)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test). The affinity constant of 67.84 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and YLEPGPVTA peptide
Catalog #MHC-HM150
Regular price $310.00
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Product Description
Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119(mB2M) and YLEPGPVTA peptide.
Product Background
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of- the MHC Class I, B2M and YLEPGPVTA peptide of- the gp100.
Product Category
Recombinant Protein
Target
HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer
MHC Form
Monomer
Allele
HLA-A*02:01
Accession
Species
Human&Mouse
Biotinylated
no
Amino Acid Range
Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and YLEPGPVTA peptide
Molecular Weight
The protein has a predicted MW of 47.90 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test). The affinity constant of 67.84 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag with an affinity constant of 67.84 nM as determined in SPR assay (Biacore T200).
Immobilized Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test).
Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer is greater than 95% as determined by SEC-HPLC.

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