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Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM150) Summary
Product Description |
Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119(mB2M) and YLEPGPVTA peptide. |
Product Background |
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of- the MHC Class I, B2M and YLEPGPVTA peptide of- the gp100. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Specifications
Protein |
HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) |
Synonyms |
HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100 |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human&Mouse |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and YLEPGPVTA peptide |
Molecular Weight |
The protein has a predicted MW of 47.90 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test). The affinity constant of 67.84 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag with an affinity constant of 67.84 nM as determined in SPR assay (Biacore T200). |
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Immobilized Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test). |
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Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer is greater than 95% as determined by SEC-HPLC. |
Additional Information
Other Available Proteins:
Finding available proteins...
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