Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A1 (KVLEYVIKV) Monomer Protein (MHC-HM103)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 58.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and KVLEYVIKV peptide
Catalog #MHC-HM103
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Product Description
 Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A1 (KVLEYVIKV) Monomer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and KVLEYVIKV peptide.
Product Background
 MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors.
Product Category
 Recombinant Protein
Target
 HLA-A*02:01 (mα3) &mB2M&MAGE-A1 (KVLEYVIKV) Monomer
Synonyms
 MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human&Mouse
Biotinylated
 no
Amino Acid Range
 Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and KVLEYVIKV peptide
Molecular Weight
 The protein has a predicted MW of 48.00 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 58.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Chimeric HLA-A*02:01(mα3)&mB2M&MAGE-A1 (KVLEYVIKV) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) TCR with an affinity constant of 58.68 nM as determined in SPR assay (Biacore T200).
Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A1 (KVLEYVIKV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC.

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