Chimeric HLA-A*02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) Monomer Protein

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 23.0ng/ml determined by ELISA (QC Test). The affinity constant of 9.32 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and SLLMWITQC peptide

Catalog #MHC-HM151

Price

$341⁰⁰

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Chimeric HLA-A*02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) Monomer Protein (MHC-HM151) Summary

Product Description	Recombinant Chimeric HLA-A02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) Monomer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and SLLMWITQC peptide.
Product Background	NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.

Protein	HLA-A*02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC)
Synonyms	MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1; CTAG1A;MY-ESO1; LAGE2; NY-ESO1
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P01887(Mouse B2M)&SLLMWITQC
Species	Human&Mouse
Biotinylated	no
Amino Acid Range	Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and SLLMWITQC peptide
Molecular Weight	The protein has a predicted MW of 48.02 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 23.0ng/ml determined by ELISA (QC Test). The affinity constant of 9.32 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Chimeric HLA-A02:01(mα3)&mB2M&NY-ESO-1(SLLMWITQC) Monomer, His Tag with an affinity constant of 9.32 nM as determined in SPR assay (Biacore T200).
	Immobilized Anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 23.0ng/ml determined by ELISA (QC Test).
	Chimeric HLA-A*02:01 (mα3) &mB2M&NY-ESO-1 (SLLMWITQC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.