Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer Protein

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer, His Tag with the EC50 of 0.47ug/ml determined by ELISA.
Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and HMTEVVRHC peptide

Catalog #MHC-HM41C

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$341.00

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Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer Protein (MHC-HM41C) Summary

Product Description	Recombinant Chimeric HLA-A02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A02:01 α1&α2), Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and HMTEVVRHC peptide.
Product Background	p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Protein	HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC)
Synonyms	MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P01887(Mouse B2M)&HMTEVVRHC
Species	Human&Mouse
Biotinylated	no
Amino Acid Range	Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and HMTEVVRHC peptide
Molecular Weight	The protein has a predicted MW of 48.04 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer, His Tag with the EC50 of 0.47ug/ml determined by ELISA.
Product Tag	C-His-Avi
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer, His Tag with the EC50 of 0.47ug/ml determined by ELISA.
	Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Chimeric HLA-A02:01 (mα3) &mB2M&P53 R175H (HLA-A02:01), His Tag on Anti-His-Biosensor can bind Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody , hFc Tag with an affinity constant of 3.32 nM as determined in BLI assay .
	The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer is greater than 90% as determined by SEC-HPLC.

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