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Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer Protein (MHC-HM41C) Summary
Product Description |
Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2), Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and HMTEVVRHC peptide. |
Product Background |
p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Specifications
Protein |
HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) |
Synonyms |
MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53 |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human&Mouse |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and HMTEVVRHC peptide |
Molecular Weight |
The protein has a predicted MW of 48.04 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer, His Tag with the EC50 of 0.47ug/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer, His Tag with the EC50 of 0.47ug/ml determined by ELISA. |
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Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HLA-A*02:01), His Tag on Anti-His-Biosensor can bind Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody , hFc Tag with an affinity constant of 3.32 nM as determined in BLI assay . |
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The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&P53 R175H (HMTEVVRHC) Monomer is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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