Chimeric HLA-A*02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer Protein (MHC-HM107)
Chimeric HLA-A*02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer Protein (MHC-HM107)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and ILAKFLHWL peptide
Catalog #MHC-HM107
Regular price $310.00
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Product Description

Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and ILAKFLHWL peptide.

Product Background

The human telomerase catalytic subunit human telomerase reverse transcriptase (hTERT) is expressed in many human cancer cells derived from multiple tissues, but infrequently in normal cells. Thus, hTERT is an attractive candidate target for tumor immunotherapy. hTERT:540–548 peptide (p540, ILAKFLHWL) has been identified as an effective HLA-A*0201-restricted T-cell epitope, an effective T-cell-based cancer treatment, including vaccines, against hTERT will likely require the identification of other MHC class I-restricted epitopes in this antigen.

Product Category

Recombinant Protein / Antibody discovery / MHC

Protein

HLA-A*02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer

Synonyms

TERT; ILAKFLHWL

MHC Form

Monomer

Allele

HLA-A*02:01

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and ILAKFLHWL peptide

Molecular Weight

The protein has a predicted MW of 48.00 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Chimeric HLA-A*02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Chimeric HLA-A*02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&TERT (ILAKFLHWL) Monomer is greater than 95% as determined by SEC-HPLC.

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