Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HM467)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer, His Tag with the EC50 of 39.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and RMFPNAPYL peptide
Catalog #MHC-HM467
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Product Description
 Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2), Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and RMFPNAPYL peptide.
Product Background
 The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1.
Product Category
 Recombinant Protein
Target
 HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human&Mouse
Biotinylated
 no
Amino Acid Range
 Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and RMFPNAPYL peptide
Molecular Weight
 The protein has a predicted MW of 48.04 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer, His Tag with the EC50 of 39.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer, His Tag with the EC50 of 39.6ng/ml determined by ELISA.
Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC.

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