Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HM467)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer, His Tag with the EC50 of 39.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and RMFPNAPYL peptide

Catalog: MHC-HM467

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Product Description Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2), Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and RMFPNAPYL peptide.

Product Background

The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1.

Product Category

Recombinant Protein

Target

HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer
Accession
MHC Form Monomer
Allele HLA-A*02:01

Species

Human&Mouse

Amino Acid Range

Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and RMFPNAPYL peptide
Molecular Weight The protein has a predicted MW of 48.04 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer, His Tag with the EC50 of 39.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer, His Tag with the EC50 of 39.6ng/ml determined by ELISA.
The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC.
Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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