Cynomolgus 4-1BB Ligand/TNFSF9 Protein (BBL-CM241)
Cynomolgus 4-1BB Ligand/TNFSF9 Protein (BBL-CM241)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque 4-1BB, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg68-Glu251
Catalog #BBL-CM241
Regular price $346.50
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Product Description

Recombinant Cynomolgus 4-1BB Ligand/TNFSF9 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Arg68-Glu251.

Product Background

The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

4-1BB Ligand/TNFSF9

Synonyms

41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9

Accession

Species

Cynomolgus

Biotinylated

no

Amino Acid Range

Arg68-Glu251

Molecular Weight

The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 50-52 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Cynomolgus/Rhesus macaque 4-1BB, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus/Rhesus macaque 4-1BB, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. Immobilized Cynomolgus/Rhesus macaque 4-1BB, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
Cynomolgus 4-1BB Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus 4-1BB Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus 4-1BB Ligand is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus 4-1BB Ligand is greater than 95% as determined by SEC-HPLC.

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