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Cynomolgus 4-1BB Ligand/TNFSF9 Protein (BBL-CM241) Summary
Product Description |
Recombinant Cynomolgus 4-1BB Ligand/TNFSF9 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Arg68-Glu251. |
Product Background |
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Cynomolgus 4-1BB Ligand/TNFSF9 Protein (BBL-CM241) Specifications/Details
Protein |
4-1BB Ligand/TNFSF9 |
Synonyms |
41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Arg68-Glu251 |
Molecular Weight |
The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 50-52 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus/Rhesus macaque 4-1BB, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus/Rhesus macaque 4-1BB, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. |
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Cynomolgus 4-1BB Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Cynomolgus 4-1BB Ligand, hFc Tag on ProA-Biosensor can bind Cynomolgus/Rhesus macaque 4-1BB, His Tag with an affinity constant of 23.5 nM as determined in BLI assay . |
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The purity of Cynomolgus 4-1BB Ligand is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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