Cynomolgus ACE2/ACEH Protein (ACE-CM401)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus ACE2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 0.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln18-Val739

Catalog: ACE-CM401

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Product Description Recombinant Cynomolgus ACE2/ACEH Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln18-Val739.
Product Category Recombinant Protein
Target ACE2/ACEH
Synonyms ACE-2; ACEH; ACE2
Gene/Protein ID A0A2K5X283
Species Cynomolgus
Exact Sequence Gln18-Val739
Molecular Weight The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus ACE2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 0.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus ACE2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 0.9ng/ml determined by ELISA.
The purity of Cynomolgus ACE2 is greater than 95% as determined by SEC-HPLC.
Cynomolgus ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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