Name: Cynomolgus Alkaline Phosphatase (Placental type) / ALPP Protein (APE-CM102)
Brand: KactusBio
SKU: APE-CM102-100UG
Price: 346.5 USD
Availability: InStock

Cynomolgus Alkaline Phosphatase (Placental type) / ALPP Protein (APE-CM102)

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Cynomolgus Alkaline Phosphatase (Placental type) , His Tag with an affinity constant of 0.11 uM as determined in SPR assay (Biacore T200).

Cynomolgus Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail.Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 7000 pmol/min/µg.
Amino Acid Range: Ile21-Thr503

Catalog #APE-CM102

Size

100UG

500UG

Order by 4pm ET for delivery on:

[{"id":43911542276318,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"APE-CM102-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Cynomolgus Alkaline Phosphatase (Placental type) \/ ALPP Protein (APE-CM102) - 100UG","public_title":"100UG","options":["100UG"],"price":34650,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":43911551516894,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"APE-CM102-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Cynomolgus Alkaline Phosphatase (Placental type) \/ ALPP Protein (APE-CM102) - 500UG","public_title":"500UG","options":["500UG"],"price":115500,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Cynomolgus Alkaline Phosphatase (Placental type) / ALPP Protein (APE-CM102) Summary

Product Description	Recombinant Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Thr503.
Product Background	Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Cynomolgus Alkaline Phosphatase (Placental type) / ALPP Protein (APE-CM102) Specifications/Details

Protein	Alkaline Phosphatase (Placental type)/ALPP
Synonyms	Alkaline phosphatase; PLAP-1; ALPP; PLAP
Accession	XP_045223825.1
Species	Cynomolgus
Biotinylated	no
Amino Acid Range	Ile21-Thr503
Molecular Weight	The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail.Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 7000 pmol/min/µg.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Cynomolgus Alkaline Phosphatase (Placental type) , His Tag with an affinity constant of 0.11 uM as determined in SPR assay (Biacore T200).
	Cynomolgus Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.