Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (APE-CM102)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ile21-Thr503

Catalog: APE-CM102

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Product Description Recombinant Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Thr503.

Product Background

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

Product Category

Recombinant Protein

Target

Alkaline Phosphatase (Placental type) /ALPP

Gene/Protein ID

XP_045223825.1

Species

Cynomolgus

Exact Sequence

Ile21-Thr503
Molecular Weight The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Cynomolgus Alkaline Phosphatase (Placental type) , His Tag with an affinity constant of 0.11 uM as determined in SPR assay (Biacore T200).
Cynomolgus Alkaline Phosphatase (Placental type) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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