Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (APE-CM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ile21-Thr503
Catalog #APE-CM102
Regular price $315.00
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Product Description

Recombinant Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Thr503.

Product Background

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

Product Category

Recombinant Protein / Antibody discovery

Protein

Alkaline Phosphatase (Placental type) /ALPP

Synonyms

Alkaline phosphatase; PLAP-1; ALPP; PLAP

Accession

Species

Cynomolgus

Biotinylated

no

Amino Acid Range

Ile21-Thr503

Molecular Weight

The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Cynomolgus Alkaline Phosphatase (Placental type) , His Tag with an affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Cynomolgus Alkaline Phosphatase (Placental type) , His Tag with an affinity constant of 0.11 uM as determined in SPR assay (Biacore T200).
Cynomolgus Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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support@kactusbio.us

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