Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (APE-CM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ile21-Thr503
Catalog #APE-CM102
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Product Description
 Recombinant Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Thr503.
Product Background
 Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product Category
 Recombinant Protein
Target
 Alkaline Phosphatase (Placental type) /ALPP
Synonyms
 Alkaline phosphatase; PLAP-1; ALPP; PLAP
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ile21-Thr503
Molecular Weight
 The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.11 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Cynomolgus Alkaline Phosphatase (Placental type) , His Tag with an affinity constant of 0.11 uM as determined in SPR assay (Biacore T200).
Cynomolgus Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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