Cynomolgus ANGPTL3/Angiopoietin-like 3 Protein (ANG-CM103)
Cynomolgus ANGPTL3/Angiopoietin-like 3 Protein (ANG-CM103)
Cynomolgus ANGPTL3/Angiopoietin-like 3 Protein (ANG-CM103)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ser17-Glu460
Catalog #ANG-CM103
Regular price $346.50
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Cynomolgus ANGPTL3/Angiopoietin-like 3 Protein (ANG-CM103) Summary

Product Description

Recombinant Cynomolgus ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser17-Glu460.

Product Background

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Cynomolgus ANGPTL3/Angiopoietin-like 3 Protein (ANG-CM103) Specifications/Details

Protein
ANGPTL3/Angiopoietin-like 3
Synonyms
ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Ser17-Glu460
Molecular Weight
The protein has a predicted MW of 52.86 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test).
Cynomolgus ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus ANGPTL3 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus ANGPTL3 is greater than 95% as determined by SEC-HPLC.
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