Cynomolgus APRIL/TNFSF13 Trimer Protein (APR-CM410)
Catalog: APR-CM410
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Product Description | Recombinant Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.It contains Lys112-Leu250. |
Product Background |
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFFR. |
Product Category |
Recombinant Protein |
Target |
APRIL/TNFSF13 Trimer |
Synonyms | CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik |
Accession | |
Species |
Cynomolgus |
Amino Acid Range |
Lys112-Leu250 |
Molecular Weight | The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi-Flag |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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