Cynomolgus APRIL/TNFSF13 Trimer Protein (APR-CM410)
Catalog: APR-CM410
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| Product Description | Recombinant Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.It contains Lys112-Leu250. |
| Product Category | Recombinant Protein |
| Target | APRIL/TNFSF13 Trimer |
| Synonyms | CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik |
| Gene/Protein ID | A0A2K5TJA1 |
| Species | Cynomolgus |
| Exact Sequence | Lys112-Leu250 |
| Molecular Weight | The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Avi,N-Flag |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. |
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The purity of Cynomolgus APRIL Trimer is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus APRIL Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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