Cynomolgus APRIL/TNFSF13 Trimer Protein (APR-CM410)
Cynomolgus APRIL/TNFSF13 Trimer Protein (APR-CM410)
  • Express System: HEK293
  • Product Tag: N-His-Avi-Flag
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 1.9 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys112-Leu250
Catalog #APR-CM410
Regular price $315.00
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Product Description
Recombinant Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.It contains Lys112-Leu250.
Product Background
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFFR.
Product Category
Recombinant Protein
Target
APRIL/TNFSF13 Trimer
Synonyms
CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Lys112-Leu250
Molecular Weight
The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 1.9 ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-His-Avi-Flag
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 1.9 ng/ml determined by ELISA.
Cynomolgus APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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