- Express System: HEK293
- Product Tag: N-His-Avi-Flag
- Purity: >95% as determined by Bis-Tris PAGE
- Activity: Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 1.9 ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Lys112-Leu250
Product Description |
Recombinant Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.It contains Lys112-Leu250. |
Product Background |
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFFR. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
APRIL/TNFSF13 Trimer |
Synonyms |
CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Lys112-Leu250 |
Molecular Weight |
The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 1.9 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi-Flag |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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