Cynomolgus B7-1/CD80 Protein (B71-CM180)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 9.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val35-Asn242

Catalog: B71-CM180

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Product Description Recombinant Cynomolgus B7-1/CD80 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Val35-Asn242.
Product Category Recombinant Protein
Target B7-1/CD80
Synonyms CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7
Gene/Protein ID G7NXN7
Species Cynomolgus
Exact Sequence Val35-Asn242
Molecular Weight The protein has a predicted MW of 25 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 9.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus B7-1, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 9.90 nM as determined in SPR assay (Biacore T200).
The purity of Cynomolgus B7-1 is greater than 95% as determined by SEC-HPLC.
Cynomolgus B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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