Cynomolgus B7-2/CD86 Protein (B72-CM186)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.102 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Tyr25-His239
Catalog #B72-CM186
Regular price $270.00
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Product Description
Recombinant Cynomolgus B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Tyr25-His239.
Product Background
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Product Category
Recombinant Protein
Target
B7-2/CD86
Synonyms
CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Tyr25-His239
Molecular Weight
The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.102 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus B7-2, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 0.102 uM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
Cynomolgus B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus B7-2 is greater than 95% as determined by SEC-HPLC.

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