Cynomolgus B7-2/CD86 Protein (B72-CM186)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.102 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Tyr26-His240

Catalog: B72-CM186

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Product Description	Recombinant Cynomolgus B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr26-His240.
Product Background	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Product Category	Recombinant Protein

Target	B7-2/CD86
Synonyms	CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2
Gene/Protein ID	G7NXR4
Species	Cynomolgus
Exact Sequence	Tyr26-His240
Molecular Weight	The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.102 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus B7-2, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 0.102 uM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
	The purity of Cynomolgus B7-2 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.