Name: Cynomolgus B7-2/CD86 Protein (B72-CM186)
Brand: KactusBio
SKU: B72-CM186-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.102 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Tyr25-His239

Catalog #B72-CM186

Size

100UG

500UG

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Cynomolgus B7-2/CD86 Protein (B72-CM186) Summary

Product Description	Recombinant Cynomolgus B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Tyr25-His239.
Product Background	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Cynomolgus B7-2/CD86 Protein (B72-CM186) Specifications/Details

Protein	B7-2/CD86
Synonyms	CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2
Accession	A0A2K5TTJ8
Species	Cynomolgus
Biotinylated	no
Amino Acid Range	Tyr25-His239
Molecular Weight	The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.102 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Cynomolgus B7-2, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 0.102 uM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA (QC Test).
	Cynomolgus B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Cynomolgus B7-2 is greater than 95% as determined by SEC-HPLC.

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