Cynomolgus B7-H6/NCR3LG1 Protein (BH7-CM176)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp25-Asp259
Catalog #BH7-CM176
Regular price $270.00
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Product Description
Recombinant Cynomolgus B7-H6/NCR3LG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp25-Asp259.
Product Background
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins, which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7-H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
Product Category
Recombinant Protein
Target
B7-H6/NCR3LG1
Synonyms
B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1; B7 Homolog 6
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Asp25-Asp259
Molecular Weight
The protein has a predicted MW of 27.6 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H6, His Tag with an affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
Cynomolgus B7-H6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus B7-H6 is greater than 95% as determined by SEC-HPLC.

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