Non-biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein (BAF-CM412)
Non-biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein (BAF-CM412)
Non-biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein (BAF-CM412)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Cynomolgus BAFF Trimer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of 31.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Thr141-Leu285
Catalog #BAF-CM412
Regular price $363.00
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Product Description

Recombinant Cynomolgus BAFF / TNFSF13B / CD257 Trimer Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Thr141-Leu285 trimer design.

Product Background

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
BAFF/TNFSF13B/CD257
Synonyms
BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Thr141-Leu285
Molecular Weight
The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus BAFF Trimer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of 31.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Avi
Expression System
HEK293
Purity
> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus BAFF Trimer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of 31.6ng/ml determined by ELISA. Immobilized Cynomolgus BAFF Trimer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of 31.6ng/ml determined by ELISA.
Cynomolgus BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Cynomolgus BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Cynomolgus BAFF Trimer is greater than 90% as determined by SEC-HPLC. The purity of Cynomolgus BAFF Trimer is greater than 90% as determined by SEC-HPLC.
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