ELISA binding curve showing anti-BAFFR antibody interaction with cynomolgus BAFFR protein containing His tag.
Cynomolgus/Rhesus macaque BAFFR/TNFRSF13C Protein (BAF-CM10R)
Cynomolgus/Rhesus macaque BAFFR/TNFRSF13C Protein (BAF-CM10R)
Cynomolgus/Rhesus macaque BAFFR/TNFRSF13C Protein (BAF-CM10R)
Cynomolgus/Rhesus macaque BAFFR/TNFRSF13C Protein (BAF-CM10R)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human BAFF (Trimer), hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BAFFR, His Tag with the EC50 of 3.4ng/ml determined by ELISA.
  • Amino Acid Range: Ser7-Ala71
Catalog #BAF-CM10R
Regular price $346.50
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Cynomolgus/Rhesus macaque BAFFR/TNFRSF13C Protein (BAF-CM10R) Summary

Product Description

Recombinant Cynomolgus/Rhesus macaque BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser7-Ala71.

Product Background

BAFFR is an atypical representative of the TNF-receptor super-family. BAFFR [BAFFR = B cell activating f actor of the TNF-f amily receptor; a.k.a. BR3, Bcmd] expression starts when the immature B cells develop to transitional B cells, which then receive BAFFR-dependent pro-survival signals to rescue them from premature cell death. At protein level, the BAFFR is expressed on the surface of all human peripheral B cell subsets except for plasma cells and for centroblasts located in the dark zone of germinal centers. Its expression is upregulated after the expression of functional B cell antigen receptors (BCR) in response to tonic BCR signaling, which enhances BAFFR expression by immature and transitional B cells

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Cynomolgus/Rhesus macaque BAFFR/TNFRSF13C Protein (BAF-CM10R) Specifications/Details

Protein
BAFFR/TNFRSF13C
Synonyms
CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin
Accession
Species
Cynomolgus/Rhesus macaque
Biotinylated
no
Amino Acid Range
Ser7-Ala71
Molecular Weight
The protein has a predicted MW of 7.7 kDa. Due to glycosylation, the protein migrates to 13-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human BAFF (Trimer), hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BAFFR, His Tag with the EC50 of 3.4ng/ml determined by ELISA.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human BAFF (Trimer), hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BAFFR, His Tag with the EC50 of 3.4ng/ml determined by ELISA. Immobilized Human BAFF (Trimer), hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BAFFR, His Tag with the EC50 of 3.4ng/ml determined by ELISA.
Immobilized Cynomolgus/Rhesus macaque BAFFR, His Tag at 1ug/ml (100ul/Well) on plate.Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. Immobilized Cynomolgus/Rhesus macaque BAFFR, His Tag at 1ug/ml (100ul/Well) on plate.Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
Cynomolgus/Rhesus macaque BAFFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus/Rhesus macaque BAFFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Cynomolgus/Rhesus macaque BAFFR, His Tag on Anti-His-Biosensor can bind Human BAFF (Trimer), monomeric hFc-Flag Tag with an affinity constant of 1.56 nM as determined in BLI assay (Gator® Prime). Loaded Cynomolgus/Rhesus macaque BAFFR, His Tag on Anti-His-Biosensor can bind Human BAFF (Trimer), monomeric hFc-Flag Tag with an affinity constant of 1.56 nM as determined in BLI assay (Gator® Prime).
The purity of Cynomolgus/Rhesus macaque BAFFR is greater than 90% as determined by SEC-HPLC. The purity of Cynomolgus/Rhesus macaque BAFFR is greater than 90% as determined by SEC-HPLC.
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