Cynomolgus BDCA-2 Protein (BCA-CM102)
Cynomolgus BDCA-2 Protein (BCA-CM102)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Cynomolgus BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Tyr48-Ile212
Catalog #BCA-CM102
Regular price $315.00
/
Size

Order now, ships by:

Product Description
Recombinant Cynomolgus BDCA-2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Tyr48-Ile212.
Product Background
BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.
Product Category
Recombinant Protein
Target
BDCA-2
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Tyr48-Ile212
Molecular Weight
The protein has a predicted MW of 20.55 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS, 300mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Cynomolgus BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed