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Cynomolgus BDCA-2 Protein (BCA-CM102) Summary
Product Description |
Recombinant Cynomolgus BDCA-2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Tyr48-Ile212. |
Product Background |
BDCA-2 is a novel type II C-type lectin specifically expressed by plasmacytoid dendritic cells (PDCs) that can internalize antigen for presentation to T cells. Furthermore, signaling via BDCA-2 may play a role in switching from interferon (IFN)-α/β-controlled to interleukin (IL)-12-controlled immune response pathways, as triggering of BDCA-2 potently inhibits secretion of IFN-α/β by PDCs and thereby promotes IL-12 p70 production in PDCs and other cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Cynomolgus BDCA-2 Protein (BCA-CM102) Specifications/Details
Protein |
BDCA-2 |
Synonyms |
BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303; |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Tyr48-Ile212 |
Molecular Weight |
The protein has a predicted MW of 20.55 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 300mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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|
Immobilized Cynomolgus BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. |
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Cynomolgus BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-BDCA-2 Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus BDCA-2, His Tag with an affinity constant of 3.67 nM as determined in BLI assay . |
Other Available Proteins:
Finding available proteins...
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