Cynomolgus BST1 Protein (BST-CM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Gly26-Ala289

Catalog: BST-CM101

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Product Description Recombinant Cynomolgus BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly26-Ala289.

Product Background

BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism.

Product Category

Recombinant Protein

Target

BST1

Gene/Protein ID

A0A2K5VGB5

Species

Cynomolgus

Exact Sequence

Gly26-Ala289
Molecular Weight The protein has a predicted MW of 30.99 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Cynomolgus BST1 is greater than 95% as determined by SEC-HPLC.
Cynomolgus BST1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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