Cynomolgus CD200 R1/CRTR2 Protein (CD2-CM4R1)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD200 R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). The affinity constant of 35.84 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu25-Leu267
Catalog #CD2-CM4R1
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Product Description
 Recombinant Cynomolgus CD200 R1/CRTR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Leu267.
Product Background
 CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.
Product Category
 Recombinant Protein
Target
 CD200 R1/CRTR2
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Glu25-Leu267
Molecular Weight
 The protein has a predicted MW of 28.09 kDa. Due to glycosylation, the protein migrates to 67-77 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus CD200 R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). The affinity constant of 35.84 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus CD200 R1, His Tag immobilized on CM5 Chip can bind Cynomolgus CD200, His Tag with an affinity constant of 35.84 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus CD200 R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
Cynomolgus CD200 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus CD200 R1 is greater than 95% as determined by SEC-HPLC.

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