Cynomolgus CD228/MFI2 Protein (MFI-CM128)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Gly20-Cys709

Catalog: MFI-CM128

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Product Description Recombinant Cynomolgus CD228/MFI2 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly20-Cys709.

Product Background

Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition.

Product Category

Recombinant Protein

Target

CD228/MFI2
Accession

Species

Cynomolgus

Exact Sequence

Gly20-Cys709
Molecular Weight The protein has a predicted MW of 76.39 kDa. Due to glycosylation, the protein migrates to 77-90 kDa based on Tris-Bis PAGE result.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Cynomolgus CD228 is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD228 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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