Cynomolgus CD27/TNFRSF7 Protein (CD2-CM127)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus CD27, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Thr21-Ile192

Catalog: CD2-CM127

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Product Description	Recombinant Cynomolgus CD27/TNFRSF7 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr21-Ile192.
Product Background	CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Product Category	Recombinant Protein

Target	CD27/TNFRSF7
Gene/Protein ID	XP_005569963.1
Species	Cynomolgus
Exact Sequence	Thr21-Ile192
Molecular Weight	The protein has a predicted MW of 20.41 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus CD27, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus CD27, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA.
	The purity of Cynomolgus CD27 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus CD27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.