Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein (CD3-CM2ED)

  • Express System: Expi293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD3E 1-27 peptide, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 37.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp22-Thr48

Catalog: CD3-CM2ED

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Product Description Recombinant Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal.It contains Asp22-Thr48.
Product Category Recombinant Protein
Target CD3E/CD3 epsilon 1-27
Synonyms CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Gene/Protein ID Q95LI5.2
Species Cynomolgus
Exact Sequence Asp22-Thr48
Molecular Weight The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus CD3E 1-27 peptide, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 37.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD3E 1-27 peptide, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 37.2ng/ml determined by ELISA.
The purity of Cynomolgus CD3E 1-27 peptide is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD3E 1-27 peptide on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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