Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101)
Catalog: CDE-CM101
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| Product Description | Recombinant Cynomolgus CD3E/CD3 Epsilon Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Asp117. |
| Product Category | Recombinant Protein |
| Target | CD3E/CD3 epsilon |
| Synonyms | CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
| Gene/Protein ID | Q95LI5 |
| Species | Cynomolgus |
| Exact Sequence | Gln22-Asp117 |
| Molecular Weight | The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 14-16 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 45.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 45.0ng/ml determined by ELISA. |
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The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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