Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101)
Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101)
Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln22-Asp117
Catalog #CDE-CM101
Regular price $285.00
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Product Description
Recombinant Cynomolgus CD3E/CD3 Epsilon Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Asp117.
Product Background
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Product Category
Recombinant Protein
Target
CD3E/CD3 epsilon
Synonyms
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Gln22-Asp117
Molecular Weight
The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 14-16 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
Cynomolgus CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.

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