Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-Asp117

Catalog: CDE-CM101

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Product Description Recombinant Cynomolgus CD3E/CD3 Epsilon Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Asp117.

Product Background

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Product Category

Recombinant Protein

Target

CD3E/CD3 epsilon
Synonyms CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18

Gene/Protein ID

Q95LI5

Species

Cynomolgus

Exact Sequence

Gln22-Asp117
Molecular Weight The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 14-16 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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