Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD3E, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 32.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-Asp117

Catalog: CDE-CM101

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Product Description Recombinant Cynomolgus CD3E/CD3 Epsilon Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Gln22-Asp117.
Product Category Recombinant Protein
Target CD3E/CD3 epsilon
Synonyms CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Gene/Protein ID Q95LI5-1
Species Cynomolgus
Exact Sequence Gln22-Asp117
Molecular Weight The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 14-16 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus CD3E, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 32.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD3E, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 32.7ng/ml determined by ELISA.
The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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