Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 45.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-Asp117

Catalog: CDE-CM101

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Product Description Recombinant Cynomolgus CD3E/CD3 Epsilon Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Asp117.
Product Category Recombinant Protein
Target CD3E/CD3 epsilon
Synonyms CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Gene/Protein ID Q95LI5
Species Cynomolgus
Exact Sequence Gln22-Asp117
Molecular Weight The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 14-16 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 45.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 45.0ng/ml determined by ELISA.
The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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