Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101)
Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101)
Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln22-Asp117
Catalog #CDE-CM101
Regular price $313.50
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Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101) Summary

Product Description

Recombinant Cynomolgus CD3E/CD3 Epsilon Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Asp117.

Product Background

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM101) Specifications/Details

Protein
CD3E/CD3 epsilon
Synonyms
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Gln22-Asp117
Molecular Weight
The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 14-16 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Expression System
HEK293
Purity
> 90% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus CD3E, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test).
Cynomolgus CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Cynomolgus CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
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