Cynomolgus CD3E/CD3 epsilon Protein (CDE-CM201)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Cynomolgus CD3E, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, mFc Tag with the EC50 of 39.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-Asp117

Catalog: CDE-CM201

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Product Description Recombinant Cynomolgus CD3E/CD3 epsilon Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gln22-Asp117.
Product Category Recombinant Protein
Target CD3E/CD3 epsilon
Synonyms CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Gene/Protein ID Q95LI5-1
Species Cynomolgus
Exact Sequence Gln22-Asp117
Molecular Weight The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus CD3E, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, mFc Tag with the EC50 of 39.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD3E, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, mFc Tag with the EC50 of 39.4ng/ml determined by ELISA.
The purity of Cynomolgus CD3E Protein is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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