Name: Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-CM201)
Brand: KactusBio
SKU: CD3-CM201-100UG
Price: 363.0 USD
Availability: InStock

Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-CM201)

Immobilized Cynomolgus CD3E&CD3D, hFc Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Antibody, hFc Tag with EC50 of 52.6 ng/ml determined by ELISA.

Cynomolgus CD3E&CD3D on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Cynomolgus CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus CD3E&CD3D, hFc Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Antibody, hFc Tag with EC50 of 52.6 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln22-Asp117 (CD3E) & Phe22-Ala105 (CD3D)

Catalog #CD3-CM201

Size

100UG

500UG

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Product Description	Recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc tag at the C-Terminus of CD3E and CD3D.; It contains Gln22-Asp117 (CD3E) and Phe22-Ala105 (CD3D).
Product Background	T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms	CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Accession	Q95LI5.2 (CD3E)&Q95LI8 (CD3D)
Species	Cynomolgus
Biotinylated	no
Amino Acid Range	Gln22-Asp117 (CD3E) & Phe22-Ala105 (CD3D)
Molecular Weight	The protein has a predicted MW of 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa (CD3E) and 44-46 kDa (CD3D) based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus CD3E&CD3D, hFc Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Antibody, hFc Tag with EC50 of 52.6 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Cynomolgus CD3E&CD3D, hFc Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Antibody, hFc Tag with EC50 of 52.6 ng/ml determined by ELISA.
	Cynomolgus CD3E&CD3D on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Cynomolgus CD3E&CD3D was greater than 95% as determined by SEC-HPLC.