Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-CM202)
Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-CM202)
Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-CM202)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with EC50 of 85.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)
Catalog #CD3-CM202
Regular price $330.00
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Product Description
Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117(CD3E)&Gln23-Thr113(CD3G).
Product Background
T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category
Recombinant Protein
Target
CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms
CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)
Molecular Weight
The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with EC50 of 85.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with EC50 of 85.5ng/ml determined by ELISA (QC Test).
Cynomolgus CD3E&CD3G on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Cynomolgus CD3E&CD3G was greater than 95% as determined by SEC-HPLC.

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