Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-CM202)
Catalog: CD3-CM202
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| Product Description | Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Gln22-Asp117 (CD3E)&Gln23-Thr113 (CD3G). |
| Product Category | Recombinant Protein |
| Target | CD3E&CD3G/CD3 epsilon&CD3 gamma |
| Synonyms | CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G |
| Gene/Protein ID | Q95LI5.2 (CD3E)&Q95LI7 (CD3G) |
| Species | Cynomolgus |
| Exact Sequence | Gln22-Asp117 (CD3E)&Gln23-Thr113 (CD3G) |
| Molecular Weight | The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G) . Due to glycosylation, the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 14.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 14.0ng/ml determined by ELISA. |
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Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 14.0ng/ml / 14.6ng/ml / 13.6ng/ml determined by ELISA. |
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The purity of Cynomolgus CD3E&CD3G was greater than 95% as determined by SEC-HPLC. |
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Cynomolgus CD3E&CD3G on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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