Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-CM202)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 51.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)

Catalog: CD3-CM202

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Product Description Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gln22-Asp117(CD3E)&Gln23-Thr113(CD3G).
Product Category Recombinant Protein
Target CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G
Gene/Protein ID Q95LI5.2(CD3E)&Q95LI7(CD3G)
Species Cynomolgus
Exact Sequence Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)
Molecular Weight The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 51.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 51.2ng/ml determined by ELISA.
The purity of Cynomolgus CD3E&CD3G was greater than 95% as determined by SEC-HPLC.
Cynomolgus CD3E&CD3G on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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