Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-CM202)
Catalog: CD3-CM202
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Product Description | Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117(CD3E)&Gln23-Thr113(CD3G). |
Product Category | Recombinant Protein |
Target | CD3E&CD3G/CD3 epsilon&CD3 gamma |
Synonyms | CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G |
Gene/Protein ID | Q95LI5.2(CD3E)&Q95LI7(CD3G) |
Species | Cynomolgus |
Exact Sequence | Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G) |
Molecular Weight | The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 51.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-hFc |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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