Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-CM202)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with EC50 of 20.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)

Catalog: CD3-CM202

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Product Description Recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117(CD3E)&Gln23-Thr113(CD3G).

Product Background

T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Product Category

Recombinant Protein

Target

CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G

Gene/Protein ID

Q95LI5.2(CD3E)&Q95LI7(CD3G)

Species

Cynomolgus

Exact Sequence

Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)
Molecular Weight The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with EC50 of 20.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with EC50 of 20.1ng/ml determined by ELISA.
The purity of Cynomolgus CD3E&CD3G was greater than 95% as determined by SEC-HPLC.
Cynomolgus CD3E&CD3G on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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