Cynomolgus CD44 Protein (CDX-CM144)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus CD44, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD44 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln21-Pro220 & Gln387-Ala428

Catalog: CDX-CM144

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Product Description	Recombinant Cynomolgus CD44 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Pro220 & Gln387-Ala428.
Product Background	CD44 is a hyaluronan binding cell surface signal transducing receptor that influences motility, cell survival and proliferation as well as the formation of tumor microenvironment. CD44 contains two variable regions encoded by variable exons. Alternative splicing, which is often deregulated in cancer, can produce various isoforms of CD44 with properties that may have different tissue specific effects and therefore even diverse effects on cancer progression
Product Category	Recombinant Protein

Target	CD44
Gene/Protein ID	A0A2K5VJE0
Species	Cynomolgus
Exact Sequence	Gln21-Pro220 & Gln387-Ala428
Molecular Weight	The protein has a predicted MW of 27.77 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus CD44, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD44 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus CD44, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD44 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
	The purity of Cynomolgus CD44 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus CD44 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.