Cynomolgus CD83 Protein (CD8-CM183)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD83, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr20-Glu147

Catalog: CD8-CM183

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Product Description Recombinant Cynomolgus CD83 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Glu147.

Product Background

CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.

Product Category

Recombinant Protein

Target

CD83

Gene/Protein ID

A0A2K5US85

Species

Cynomolgus

Exact Sequence

Thr20-Glu147
Molecular Weight The protein has a predicted MW of 15.43 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus CD83, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD83, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
The purity of Cynomolgus CD83 is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD83 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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