Cynomolgus CDCP1 Protein (CDC-CM102)
Cynomolgus CDCP1 Protein (CDC-CM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CDCP1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
  • Amino Acid Range: Phe30-Thr667
Catalog #CDC-CM102
Regular price $379.50
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Cynomolgus CDCP1 Protein (CDC-CM102) Summary

Product Description

Recombinant Cynomolgus CDCP1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Phe30-Thr667.

Product Background

Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Cynomolgus CDCP1 Protein (CDC-CM102) Specifications/Details

Protein
CDCP1
Synonyms
CDCP1; CD318; SIMA135; TRASK;
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Phe30-Thr667
Molecular Weight
The protein has a predicted MW of 73.15 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus CDCP1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus CDCP1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA. Immobilized Cynomolgus CDCP1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
Cynomolgus CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Cynomolgus CDCP1, His Tag with an affinity constant of 0.31 uM as determined in BLI assay (Gator® Prime). Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Cynomolgus CDCP1, His Tag with an affinity constant of 0.31 uM as determined in BLI assay (Gator® Prime).
The purity of Cynomolgus CDCP1 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus CDCP1 is greater than 95% as determined by SEC-HPLC.
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