• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus DLK1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLK1 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA.
  • Amino Acid Range: Ala24-Cys306
Catalog #DLK-CM101-UL
Price
Regular price $38115
Regular price Sale price $38115
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Size
Avantor VWR Fisher Scientific

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Cynomolgus DLK1 Protein, Ultra Low Endotoxin (DLK-CM101-UL) Summary

Product Description

 Recombinant Cynomolgus DLK1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala24-Cys306.

Product Background

 paternally inherited genetic defects of DLK1 were identified in four families with nonsyndromic CPP and a metabolic phenotype. DLK1 encodes a transmembrane protein that is important for adipose tissue homeostasis and neurogenesis and is located in the imprinted chromosome 14q32 region associated with Temple syndrome.

Cynomolgus DLK1 Protein, Ultra Low Endotoxin (DLK-CM101-UL) Specifications/Details

Protein
 DLK1
Synonyms
 pG2; FA1; DLK; DLK1; DLK-1; Pref1; secredeltin; ZOG
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ala24-Cys306
Molecular Weight
 The protein has a predicted MW of 31.26 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus DLK1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLK1 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus DLK1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLK1 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA. Immobilized Cynomolgus DLK1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLK1 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA.
Cynomolgus DLK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus DLK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus DLK1 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus DLK1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...