Regular price
$346.50
Order by 4pm ET for delivery on:
Cynomolgus DNAM-1/CD226 Protein, Ultra Low Endotoxin (DAM-CM101-UL) Summary
Product Description |
Recombinant Cynomolgus DNAM-1/CD226 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu19-Phe252. |
Product Background |
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Cynomolgus DNAM-1/CD226 Protein, Ultra Low Endotoxin (DAM-CM101-UL) Specifications/Details
Protein |
DNAM-1/CD226 |
Synonyms |
CD226 molecule; CD226; DNAM1; PTA1; TLiSA1 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Glu19-Phe252 |
Molecular Weight |
The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus DNAM-1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 1.63ug/ml determined by ELISA (QC Test). The affinity constant of 0.82 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human CD155, hFc Tag- captured on CM5 Chip via Protein A can bind Cynomolgus DNAM-1, His Tag with an affinity constant of 0.82 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Cynomolgus DNAM-1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 1.63ug/ml determined by ELISA (QC Test). |
|
|
Cynomolgus DNAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Cynomolgus DNAM-1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
