Cynomolgus E-selectin/CD62E Protein (ESE-CM101)
Cynomolgus E-selectin/CD62E Protein (ESE-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus E-selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Trp22-Pro554
Catalog #ESE-CM101
Regular price $315.00
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Product Description

Recombinant Cynomolgus E-selectin/CD62E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp22-Pro554.

Product Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Product Category

Recombinant Protein / Antibody discovery

Protein

E-Selectin/CD62E

Synonyms

CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E

Accession

Species

Cynomolgus

Biotinylated

no

Amino Acid Range

Trp22-Pro554

Molecular Weight

The protein has a predicted MW of 59.47 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Cynomolgus E-selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus E-selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA. Immobilized Cynomolgus E-selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.
Cynomolgus E-selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus E-selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus E-selectin is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus E-selectin is greater than 95% as determined by SEC-HPLC.

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