Cynomolgus EPHB2 Protein (EPH-CM102)
Catalog: EPH-CM102
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Product Description | Recombinant Cynomolgus EPHB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Pro542. |
Product Background |
EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia. |
Product Category |
Recombinant Protein |
Target |
EPHB2 |
Accession | |
Species |
Cynomolgus |
Amino Acid Range |
Val19-Pro542 |
Molecular Weight | The protein has a predicted MW of 59.09 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Cynomolgus EPHB2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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