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Cynomolgus Fc epsilon RI alpha/FCER1a Protein (FER-CM101) Summary
Product Description |
Recombinant Cynomolgus Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with His tag at the C-terminus.It contains Val29-Lys204. |
Product Background |
Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Cynomolgus Fc epsilon RI alpha/FCER1a Protein (FER-CM101) Specifications/Details
Protein |
Fc epsilon RI alpha/FCER1a |
Synonyms |
Fc-epsilon RI-alpha; FcERI; FCER1A; FCE1A |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Val29-Lys204 |
Molecular Weight |
The protein has a predicted MW of 22.02 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus Fc epsilon RI alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IgE, His Avi Tag (Cat. IGE-CM401B) with the EC50 of 8.8ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus Fc epsilon RI alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IgE, His Avi Tag (Cat. IGE-CM401B) with the EC50 of 8.8ng/ml determined by ELISA. |
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Immobilized Cynomolgus Fc epsilon RI alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. |
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Cynomolgus Fc epsilon RI alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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