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Cynomolgus FGL1 Protein, Ultra Low Endotoxin (FGL-CM211-UL) Summary
Product Description |
Recombinant Cynomolgus FGL1 Protein is expressed from HEK293 with hFc (IgG1) at the N-Terminus.It contains Leu23-Ile312. |
Product Background |
Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus FGL1 Protein, Ultra Low Endotoxin (FGL-CM211-UL) Specifications/Details
Protein |
FGL1 |
Synonyms |
FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Leu23-Ile312 |
Molecular Weight |
The protein has a predicted MW of 55.5 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human LAG3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus FGL1, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA (QC Test). |
Product Tag |
N-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human LAG3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus FGL1, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA (QC Test). |
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Cynomolgus FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus FGL1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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