Product Description |
Recombinant Cynomolgus Galectin 3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ala2-Ile248. |
Product Background |
Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). |
Product Category |
Recombinant Protein |
Target |
Galectin 3 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Ala2-Ile248 |
Molecular Weight |
The protein has a predicted MW of 27.05 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus Galectin 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM Hepes, 150mM NaCl, 200mM L-arginine, 2mM Tcep (pH 6.5). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Cynomolgus Galectin 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. |
|
|
Cynomolgus Galectin 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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