Cynomolgus Galectin 3 Protein (GLT-CM103)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Cynomolgus Galectin 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 19.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala2-Ile248

Catalog: GLT-CM103

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Product Description	Recombinant Cynomolgus Galectin 3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ala2-Ile248.
Product Background	Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
Product Category	Recombinant Protein

Target	Galectin 3
Gene/Protein ID	XP_005561371.1
Species	Cynomolgus
Exact Sequence	Ala2-Ile248
Molecular Weight	The protein has a predicted MW of 27.05 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus Galectin 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 19.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Hepes, 150mM NaCl, 200mM L-arginine, 2mM Tcep (pH 6.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus Galectin 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 19.5ng/ml determined by ELISA.
	Cynomolgus Galectin 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.