Cynomolgus GFRAL/GFR alpha-like Protein (GFL-CM401)
Catalog: GFL-CM401
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| Product Description | Recombinant Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu351. |
Product Background |
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. |
Product Category |
Recombinant Protein |
Target |
GFRAL/GFR alpha-like |
| Synonyms | GFR alpha-like; GFRAL; GRAL; C6orf144 |
Gene/Protein ID |
XP_015304775.2 |
Species |
Cynomolgus |
Exact Sequence |
Gln20-Glu351 |
| Molecular Weight | The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM PB, 0.4M NaCl (pH 6.0). Normally 8% mannitol is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM PB, 0.4M NaCl (pH 6.0). |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. |
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The purity of Cynomolgus GFRAL is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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