Non-biotinylated Cynomolgus GFRAL/GFR alpha-like Protein (GFL-CM401)
Non-biotinylated Cynomolgus GFRAL/GFR alpha-like Protein (GFL-CM401)
Non-biotinylated Cynomolgus GFRAL/GFR alpha-like Protein (GFL-CM401)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln20-Glu351
Catalog #GFL-CM401
Regular price $346.50
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Product Description

Recombinant Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu351.

Product Background

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
GFRAL/GFR alpha-like
Synonyms
GFR alpha-like; GFRAL; GRAL; C6orf144; GFR alpha like
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Gln20-Glu351
Molecular Weight
The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM PB, 0.4M NaCl (pH 6.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 20mM PB, 0.4M NaCl (pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Cynomolgus GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus GFRAL is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus GFRAL is greater than 95% as determined by SEC-HPLC.
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