Cynomolgus GFRAL/GFR alpha-like Protein (GFL-CM401)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln20-Glu351

Catalog: GFL-CM401

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Product Description Recombinant Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu351.

Product Background

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Product Category

Recombinant Protein

Target

GFRAL/GFR alpha-like
Synonyms GFR alpha-like; GFRAL; GRAL; C6orf144
Accession

Species

Cynomolgus

Amino Acid Range

Gln20-Glu351
Molecular Weight The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM PB, 0.4M NaCl (pH 6.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM PB, 0.4M NaCl (pH 6.0).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
The purity of Cynomolgus GFRAL is greater than 95% as determined by SEC-HPLC.
Cynomolgus GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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