Cynomolgus GITR Ligand/TNFSF18 Protein (FSF-CM118)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln72-Ser199
Catalog #FSF-CM118
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Product Description
 Recombinant Cynomolgus GITR Ligand/TNFSF18 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln72-Ser199.
Product Background
 B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Product Category
 Recombinant Protein
Target
 GITR Ligand/TNFSF18
Synonyms
 TNFSF18; AITRL; TL6; GITRL; GITR Ligand
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Gln72-Ser199
Molecular Weight
 The protein has a predicted MW of 15.62 kDa. Due to glycosylation, the protein migrates to 17-27 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus GITR Ligand, His Tag immobilized on CM5 Chip can bind Cynomolgus GITR, His Tag with an affinity constant of 0.21 uM as determined in SPR assay (Biacore T200).
Cynomolgus GITR Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus GITR Ligand is greater than 95% as determined by SEC-HPLC.

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