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Cynomolgus GITR Ligand/TNFSF18 Protein (FSF-CM118) Summary
Product Description |
Recombinant Cynomolgus GITR Ligand/TNFSF18 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln72-Ser199. |
Product Background |
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Cynomolgus GITR Ligand/TNFSF18 Protein (FSF-CM118) Specifications/Details
Protein |
GITR Ligand/TNFSF18 |
Synonyms |
TNFSF18; AITRL; TL6; GITRL; GITR Ligand |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln72-Ser199 |
Molecular Weight |
The protein has a predicted MW of 15.62 kDa. Due to glycosylation, the protein migrates to 17-27 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Cynomolgus GITR Ligand, His Tag immobilized on CM5 Chip can bind Cynomolgus GITR, His Tag with an affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). |
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Cynomolgus GITR Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus GITR Ligand is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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