Cynomolgus GITR/TNFRSF18 Protein (GTR-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln20-Pro156
Catalog #GTR-CM101
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Product Description
 Recombinant Cynomolgus GITR/TNFRSF18 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln20-Pro156.
Product Background
 GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Product Category
 Recombinant Protein
Target
 GITR/TNFRSF18
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Gln20-Pro156
Molecular Weight
 The protein has a predicted MW of 15.87 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus GITR Ligand, His Tag immobilized on CM5 Chip can bind Cynomolgus GITR, His Tag with an affinity constant of 0.21 uM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA (QC Test).
Cynomolgus GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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