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Cynomolgus GITR/TNFRSF18 Protein (GTR-CM101) Summary
Product Description |
Recombinant Cynomolgus GITR/TNFRSF18 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln20-Pro156. |
Product Background |
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Cynomolgus GITR/TNFRSF18 Protein (GTR-CM101) Specifications/Details
Protein |
GITR/TNFRSF18 |
Synonyms |
CD357; GITR; GITR-D; TNFRSF18; AITR |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln20-Pro156 |
Molecular Weight |
The protein has a predicted MW of 15.87 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Cynomolgus GITR Ligand, His Tag immobilized on CM5 Chip can bind Cynomolgus GITR, His Tag with an affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). |
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Cynomolgus GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus GITR is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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