- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
- Activity: Immobilized Cynomolgus GPVI, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Gln21-Asn251
Product Description |
Recombinant Cynomolgus GPVI Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Gln21-Asn251. |
Product Background |
Although platelets are best known for their role in hemostasis, they are also crucial in development, host defense, inflammation, and tissue repair. Many of these roles are regulated by the immune-like receptors glycoprotein VI (GPVI) and C-type lectin receptor 2 (CLEC-2), which signal through an immunoreceptor tyrosine-based activation motif (ITAM). GPVI is activated by collagen in the subendothelial matrix, by fibrin and fibrinogen in the thrombus, and by a remarkable number of other ligands. |
Product Category |
Recombinant Protein |
Target |
GPVI |
Synonyms |
GPVI; Gp6; Glycoprotein 6; MGC138168 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln21-Asn251 |
Molecular Weight |
The protein has a predicted MW of 26.42 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus GPVI, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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