Cynomolgus HGF R/c-MET Protein, Ultra Low Endotoxin (MET-CM101-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus HGF R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Glu25-Asn930
Catalog #MET-CM101-UL
Regular price $381.00
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Product Description

Recombinant Cynomolgus HGF R/c-MET Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Asn930.

Product Background

c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

HGF R/c-MET

Synonyms

MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor

Accession

Species

Cynomolgus

Biotinylated

no

Amino Acid Range

Glu25-Asn930

Molecular Weight

The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 69.9 kDa (β chain). Due to glycosylation, the protein migrates to 45-52 kDa (α chain) and 75-82 kDa (β chain) based on Bis-Tris PAGE result.

Activity

Immobilized Cynomolgus HGF R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus HGF R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus HGF R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test).
Cynomolgus HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus HGF R is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus HGF R is greater than 95% as determined by SEC-HPLC.

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