Cynomolgus IFN gamma/IFNG Protein (IFN-CM10G)
Catalog: IFN-CM10G
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Product Description | Recombinant Cynomolgus IFN gamma/IFNG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln24-Gln165. |
Product Background |
IFN-gamma and one of its receptor subunits, IFNGR1, are translocated to the nucleus, together with STAT1alpha as one macromolecular complex, via the classical importin-dependent pathway.Put IFN-gamma and its receptor subunit, IFNGR1, in direct contact with the promoter region of IFN-gamma-activated genes with associated increased activity, thus suggesting a transcriptional/cotranscriptional role for IFN-gamma/IFNGR1 as well as a possible role in determining the specificity of IFN-gamma action. |
Product Category |
Recombinant Protein |
Target |
IFN gamma/IFNG |
Accession | |
Species |
Cynomolgus |
Amino Acid Range |
Gln24-Gln165 |
Molecular Weight | The protein has a predicted MW of 17.72 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Cynomolgus IFN gamma, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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