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Cynomolgus IFN gamma/IFNG Protein (IFN-CM10G) Summary
Product Description |
Recombinant Cynomolgus IFN gamma/IFNG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln24-Gln165. |
Product Background |
IFN-gamma and one of its receptor subunits, IFNGR1, are translocated to the nucleus, together with STAT1alpha as one macromolecular complex, via the classical importin-dependent pathway.Put IFN-gamma and its receptor subunit, IFNGR1, in direct contact with the promoter region of IFN-gamma-activated genes with associated increased activity, thus suggesting a transcriptional/cotranscriptional role for IFN-gamma/IFNGR1 as well as a possible role in determining the specificity of IFN-gamma action. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus IFN gamma/IFNG Protein (IFN-CM10G) Specifications/Details
Protein |
IFN gamma/IFNG |
Synonyms |
IFN-gamma-R1; CDw119; CD119; IFNGR1; IFNGR; AVP; FLJ45734; IFN-gamma R1; IFN-gR1; type 2; IFNγ; IFN γ |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln24-Gln165 |
Molecular Weight |
The protein has a predicted MW of 17.72 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IFN gamma, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus IFN gamma, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA. |
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Cynomolgus IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus IFN gamma is greater than 95% as determined by SEC-HPLC. |
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